TOMM20
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATP5F1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- extracellular vesicular exosome [IDA]
- membrane [IDA]
- mitochondrial inner membrane [TAS]
- mitochondrial matrix [NAS]
- mitochondrial proton-transporting ATP synthase complex [IDA]
- mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [IBA]
- mitochondrion [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATP5F1 TOMM20 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.769 | BioGRID | 753157 |
Curated By
- BioGRID