BAIT
TOMM20
MAS20, MOM19, TOM20, RP4-597N16.2
translocase of outer mitochondrial membrane 20 homolog (yeast)
GO Process (3)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GLI3
ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
GLI family zinc finger 3
GO Process (16)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- T cell differentiation in thymus [ISS]
- embryonic digestive tract development [TAS]
- embryonic digit morphogenesis [TAS]
- limb morphogenesis [IMP]
- negative regulation of alpha-beta T cell differentiation [ISS]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of smoothened signaling pathway [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative thymic T cell selection [ISS]
- nose morphogenesis [TAS]
- positive regulation of alpha-beta T cell differentiation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [ISS]
- smoothened signaling pathway [TAS]
- thymocyte apoptotic process [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID