BAIT
TOMM20
MAS20, MOM19, TOM20, RP4-597N16.2
translocase of outer mitochondrial membrane 20 homolog (yeast)
GO Process (3)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MAVS
CARDIF, IPS-1, IPS1, VISA
mitochondrial antiviral signaling protein
GO Process (23)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- activation of innate immune response [IMP]
- cellular response to exogenous dsRNA [IMP]
- defense response to bacterium [IMP]
- defense response to virus [IDA]
- innate immune response [IMP, TAS]
- negative regulation of type I interferon production [TAS]
- negative regulation of viral genome replication [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- positive regulation of IP-10 production [IDA]
- positive regulation of chemokine (C-C motif) ligand 5 production [IDA]
- positive regulation of defense response to virus by host [IDA, IMP]
- positive regulation of interferon-alpha production [IDA, IMP]
- positive regulation of interferon-beta production [IDA, IMP]
- positive regulation of interleukin-8 production [IDA]
- positive regulation of protein import into nucleus, translocation [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of tumor necrosis factor production [IDA]
- positive regulation of type I interferon-mediated signaling pathway [IDA]
- regulation of peroxisome organization [IMP]
- signal transduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID