BAIT
TOMM20
MAS20, MOM19, TOM20, RP4-597N16.2
translocase of outer mitochondrial membrane 20 homolog (yeast)
GO Process (3)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SMG7
C1orf16, EST1C, SGA56M, RP1-127C7.1
SMG7 nonsense mediated mRNA decay factor
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID