BAIT

COX14

C12orf62
COX14 cytochrome c oxidase assembly factor
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

NDUFA9

CC6, CI-39k, CI39k, NDUFS2L, SDR22E1
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.

Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Oehman T, Varjosalo M

Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]

Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]

Throughput

  • High Throughput

Curated By

  • BioGRID