BAIT
SFXN1
sideroflexin 1
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
GLRX2
CGI-133, GRX2, RP11-101E13.4
glutaredoxin 2
GO Process (12)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- DNA protection [NAS]
- apoptotic process [NAS]
- cell differentiation [NAS]
- cell redox homeostasis [TAS]
- glutathione metabolic process [TAS]
- protein folding [TAS]
- regulation of signal transduction [NAS]
- regulation of transcription, DNA-templated [NAS]
- response to hydrogen peroxide [IDA]
- response to organic substance [IDA]
- response to redox state [TAS]
- response to temperature stimulus [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID