BAIT
COX4I1
COX4, COX4-1, COXIV
cytochrome c oxidase subunit IV isoform 1
GO Process (5)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TRIAP1
MDM35, P53CSV, WF-1, HSPC132
TP53 regulated inhibitor of apoptosis 1
GO Process (11)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator [IDA]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- cellular response to UV [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- negative regulation of release of cytochrome c from mitochondria [IMP]
- phospholipid transport [IDA]
- positive regulation of phospholipid transport [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of membrane lipid distribution [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID