An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.

Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Oehman T, Varjosalo M

Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]

Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HK1 HK1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Rosano C (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

fructokinase activity [IBA]glucokinase activity [IBA]hexokinase activity [TAS]mannokinase activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

HK1