An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.

Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianferani S, Audebert S, Verdier-Pinard P, Badache A

Control of microtubule dynamics underlies several fundamental processes such as cell polarity, cell division, and cell motility. To gain insights into the mechanisms that control microtubule dynamics during cell motility, we investigated the interactome of the microtubule plus-end-binding protein end-binding 1 (EB1). Via molecular mapping and cross-linking mass spectrometry we identified and characterized a large complex associating a specific isoform ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2016; 114(50);E10687-E10696 [Pubmed: 29162697]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DYNLL1 PDE4DIP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3354853
DYNLL1 PDE4DIP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1449579

Curated By

BioGRID

Interaction Summary

PDE4DIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

DYNLL1