An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.

Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianferani S, Audebert S, Verdier-Pinard P, Badache A

Control of microtubule dynamics underlies several fundamental processes such as cell polarity, cell division, and cell motility. To gain insights into the mechanisms that control microtubule dynamics during cell motility, we investigated the interactome of the microtubule plus-end-binding protein end-binding 1 (EB1). Via molecular mapping and cross-linking mass spectrometry we identified and characterized a large complex associating a specific isoform ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2016; 114(50);E10687-E10696 [Pubmed: 29162697]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

MAPRE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule plus-end binding [IDA]poly(A) RNA binding [IDA]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

CTTN