An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.

Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianferani S, Audebert S, Verdier-Pinard P, Badache A

Control of microtubule dynamics underlies several fundamental processes such as cell polarity, cell division, and cell motility. To gain insights into the mechanisms that control microtubule dynamics during cell motility, we investigated the interactome of the microtubule plus-end-binding protein end-binding 1 (EB1). Via molecular mapping and cross-linking mass spectrometry we identified and characterized a large complex associating a specific isoform ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2016; 114(50);E10687-E10696 [Pubmed: 29162697]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAPRE1 KIAA1217	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bouguenina H (2017)	High	-	BioGRID	-
MAPRE1 KIAA1217	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3359998
MAPRE1 KIAA1217	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	102.08	BioGRID	2995738

Curated By

BioGRID

Interaction Summary

MAPRE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule plus-end binding [IDA]poly(A) RNA binding [IDA]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

KIAA1217