CKAP4
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DKK1
Gene Ontology Biological Process
- extracellular negative regulation of signal transduction [IDA]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of canonical Wnt signaling pathway [IDA, IGI]
- negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [IDA]
- negative regulation of cardiac muscle cell differentiation [IDA]
- negative regulation of mesodermal cell fate specification [IDA]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of protein complex assembly [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway [ISS]
- regulation of endodermal cell fate specification [IDA]
- regulation of receptor internalization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CKAP4 is a Dickkopf1 receptor and is involved in tumor progression.
Dickkopf1 (DKK1) is a secretory protein that antagonizes oncogenic Wnt signaling by binding to the Wnt coreceptor low-density lipoprotein receptor-related protein 6 (LRP6). DKK1 may also regulate its own signaling to promote cancer cell proliferation, but the mechanism is not understood. Here, we identified cytoskeleton-associated protein 4 (CKAP4) as a DKK1 receptor and evaluated CKAP4-mediated DKK1 signaling in cancer cell ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
DKK1 CKAP4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
CKAP4 DKK1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID