BAIT
NEK2
HsPK21, NEK2A, NLK1, PPP1R111, RP67
NIMA-related kinase 2
GO Process (12)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- centrosome separation [IDA]
- chromosome segregation [IDA]
- mitotic cell cycle [TAS]
- mitotic nuclear division [TAS]
- negative regulation of centriole-centriole cohesion [IMP]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA, IMP]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of mitosis [TAS]
- regulation of mitotic centrosome separation [TAS]
- spindle assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MYO5A
GS1, MYH12, MYO5, MYR12
myosin VA (heavy chain 12, myoxin)
GO Process (10)
GO Function (3)
GO Component (17)
Gene Ontology Biological Process
- actin filament-based movement [NAS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- membrane organization [TAS]
- post-Golgi vesicle-mediated transport [IMP]
- protein localization to plasma membrane [ISS]
- regulation of Golgi organization [IMP]
- transport [NAS]
- vesicle transport along actin filament [IMP]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [IDA]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [IDA]
- filopodium tip [IDA]
- growth cone [NAS]
- insulin-responsive compartment [ISS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- neuron projection [NAS]
- peroxisome [IDA]
- recycling endosome [IDA]
- ruffle [IDA]
- vesicle [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Destabilizing NEK2 overcomes resistance to proteasome inhibition in multiple myeloma.
Drug resistance remains the key problem in cancer treatment. It is now accepted that each myeloma patient harbors multiple subclones and subclone dominance may change over time. The coexistence of multiple subclones with high or low chromosomal instability (CIN) signature causes heterogeneity and drug resistance with consequent disease relapse. In this study, using a tandem affinity purification-mass spectrometry (TAP-MS) technique, ... [more]
J. Clin. Invest. Jul. 02, 2018; 128(7);2877-2893 [Pubmed: 29863498]
Throughput
- High Throughput
Curated By
- BioGRID