BAIT
NEK2
HsPK21, NEK2A, NLK1, PPP1R111, RP67
NIMA-related kinase 2
GO Process (12)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- centrosome separation [IDA]
- chromosome segregation [IDA]
- mitotic cell cycle [TAS]
- mitotic nuclear division [TAS]
- negative regulation of centriole-centriole cohesion [IMP]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA, IMP]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of mitosis [TAS]
- regulation of mitotic centrosome separation [TAS]
- spindle assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
APOD
apolipoprotein D
GO Process (20)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- aging [NAS]
- angiogenesis [NAS]
- brain development [ISS]
- glucose metabolic process [IDA]
- lipid metabolic process [IDA]
- lipid transport [NAS]
- negative regulation of T cell migration [IDA]
- negative regulation of cytokine production involved in inflammatory response [IDA]
- negative regulation of focal adhesion assembly [IMP]
- negative regulation of lipoprotein lipid oxidation [IDA]
- negative regulation of monocyte chemotactic protein-1 production [IDA]
- negative regulation of platelet-derived growth factor receptor signaling pathway [IDA]
- negative regulation of protein import into nucleus [IDA]
- negative regulation of smooth muscle cell proliferation [IDA]
- negative regulation of smooth muscle cell-matrix adhesion [IMP]
- peripheral nervous system axon regeneration [ISS]
- response to axon injury [ISS]
- response to drug [ISS]
- response to reactive oxygen species [IDA]
- tissue regeneration [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Destabilizing NEK2 overcomes resistance to proteasome inhibition in multiple myeloma.
Drug resistance remains the key problem in cancer treatment. It is now accepted that each myeloma patient harbors multiple subclones and subclone dominance may change over time. The coexistence of multiple subclones with high or low chromosomal instability (CIN) signature causes heterogeneity and drug resistance with consequent disease relapse. In this study, using a tandem affinity purification-mass spectrometry (TAP-MS) technique, ... [more]
J. Clin. Invest. Jul. 02, 2018; 128(7);2877-2893 [Pubmed: 29863498]
Throughput
- High Throughput
Curated By
- BioGRID