BAIT

SLC1A1

DCBXA, EAAC1, EAAT3, SCZD18, RP11-6J24.1
solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Homo sapiens
PREY

PRKCA

AAG6, PKC-alpha, PKCA, PRKACA
protein kinase C, alpha
GO Process (40)
GO Function (5)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phorbol myristate acetate-dependent interaction of protein kinase Calpha and the neuronal glutamate transporter EAAC1.

Gonzalez MI, Bannerman PG, Robinson MB

Sodium-dependent transporters clear extracellular glutamate in the mammalian CNS. Activation of protein kinase C (PKC) rapidly increases the activity of the neuronal glutamate transporter EAAC1 (excitatory amino acid carrier-1). This effect is associated with redistribution of EAAC1 to the cell membrane and appears to be dependent on a particular PKC subtype, PKCalpha. In the present study, we sought to determine ... [more]

J. Neurosci. Jul. 02, 2003; 23(13);5589-93 [Pubmed: 12843260]

Throughput

  • Low Throughput

Curated By

  • BioGRID