BAIT
VCP
ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Gene Ontology Biological Process
- DNA repair [NAS]
- ER-associated ubiquitin-dependent protein catabolic process [IDA, IMP, TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- cellular response to DNA damage stimulus [IDA]
- double-strand break repair [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment of protein localization [TAS]
- positive regulation of Lys63-specific deubiquitinase activity [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein K63-linked deubiquitination [IDA]
- positive regulation of protein catabolic process [IDA]
- positive regulation of protein complex assembly [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [NAS]
- protein N-linked glycosylation via asparagine [IMP]
- protein ubiquitination [IDA, NAS]
- regulation of apoptotic process [TAS]
- retrograde protein transport, ER to cytosol [IDA]
- translesion synthesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [ISS]
- lipid particle [IDA]
- nucleoplasm [IDA]
- nucleus [IDA, TAS]
- perinuclear region of cytoplasm [IDA]
- proteasome complex [IDA]
- site of double-strand break [IDA]
Homo sapiens
PREY
STK3
KRS1, MST2
serine/threonine kinase 3
GO Process (13)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- apoptotic process [TAS]
- hippo signaling [IDA, TAS]
- intracellular signal transduction [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of apoptotic process [ISS]
- positive regulation of fat cell differentiation [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IGI]
- protein phosphorylation [IDA]
- protein stabilization [IMP]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches.
Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes. While components of the mammalian RQC have been examined in vitro, a systematic characterization of RQC protein interactions in mammalian cells has yet to be described. Here we utilize both proximity-labeling proteomic approaches, BioID and APEX, ... [more]
Mol. Biol. Cell Dec. 15, 2017; 29(10);1258-1269 [Pubmed: 29540532]
Throughput
- High Throughput
Additional Notes
- The 'hit' protein was identified as a mass spectrometry-identified VCP-interactor in an APEX2-based proximity labeling experiment but not in a BioID-based proximity labeling experiment or in a FLAG tag immunoprecipitation.
Curated By
- BioGRID