BAIT
LTN1
C21orf10, C21orf98, RNF160, ZNF294, HSPC087
listerin E3 ubiquitin protein ligase 1
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
MYH10
NMMHC-IIB, NMMHCB
myosin, heavy chain 10, non-muscle
GO Process (5)
GO Function (7)
GO Component (13)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches.
Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes. While components of the mammalian RQC have been examined in vitro, a systematic characterization of RQC protein interactions in mammalian cells has yet to be described. Here we utilize both proximity-labeling proteomic approaches, BioID and APEX, ... [more]
Mol. Biol. Cell Dec. 15, 2017; 29(10);1258-1269 [Pubmed: 29540532]
Throughput
- High Throughput
Additional Notes
- The 'hit' protein was identified by mass spectrometry after affinity purification of Ltn1 complexes.
Curated By
- BioGRID