Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.

Watanabe S, Fujiyama H, Takafuji T, Kayama K, Matsumoto M, Nakayama KI, Yoshida K, Sugimoto N, Fujita M

Glutamate-rich WD40 repeat-containing 1 (GRWD1) is a Cdt1-binding protein that promotes mini-chromosome maintenance (MCM) loading through its histone chaperone activity. GRWD1 acts as a tumor-promoting factor by downregulating p53 (also known as TP53) via the RPL11-MDM2-p53 axis. Here, we identified GRWD1-interacting proteins using a proteomics approach and showed that GRWD1 interacts with various proteins involved in transcription, translation, DNA replication ... [more]

J. Cell. Sci. Aug. 03, 2018; 131(15); [Pubmed: 29991511]

Throughput

  • High Throughput

Additional Notes

  • The 'hit' proteins were identified in GRWD1 immunoprecipitations performed in low but not high salt.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
XPO1 GRWD1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
1439864

Curated By

  • BioGRID