BAIT
GRWD1
CDW4, GRWD, RRB1, WDR28
glutamate-rich WD repeat containing 1
GO Process (0)
GO Function (1)
GO Component (3)
Homo sapiens
PREY
CAD
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
GO Process (11)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- 'de novo' pyrimidine nucleobase biosynthetic process [IDA, ISS]
- arginine biosynthetic process [IBA]
- drug metabolic process [ISS]
- glutamine metabolic process [ISS]
- nucleobase-containing small molecule metabolic process [TAS]
- peptidyl-threonine phosphorylation [ISS]
- protein autophosphorylation [ISS]
- pyrimidine nucleobase metabolic process [TAS]
- pyrimidine nucleoside biosynthetic process [TAS]
- small molecule metabolic process [TAS]
- urea cycle [IBA]
Gene Ontology Molecular Function- ATP binding [ISS]
- aspartate binding [ISS]
- aspartate carbamoyltransferase activity [IBA, ISS, TAS]
- carbamoyl-phosphate synthase (ammonia) activity [IBA]
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [IBA, ISS, TAS]
- dihydroorotase activity [IDA, ISS, TAS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein kinase activity [ISS]
- zinc ion binding [IDA]
- ATP binding [ISS]
- aspartate binding [ISS]
- aspartate carbamoyltransferase activity [IBA, ISS, TAS]
- carbamoyl-phosphate synthase (ammonia) activity [IBA]
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [IBA, ISS, TAS]
- dihydroorotase activity [IDA, ISS, TAS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein kinase activity [ISS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.
Glutamate-rich WD40 repeat-containing 1 (GRWD1) is a Cdt1-binding protein that promotes mini-chromosome maintenance (MCM) loading through its histone chaperone activity. GRWD1 acts as a tumor-promoting factor by downregulating p53 (also known as TP53) via the RPL11-MDM2-p53 axis. Here, we identified GRWD1-interacting proteins using a proteomics approach and showed that GRWD1 interacts with various proteins involved in transcription, translation, DNA replication ... [more]
J. Cell. Sci. Aug. 03, 2018; 131(15); [Pubmed: 29991511]
Throughput
- High Throughput
Additional Notes
- The 'hit' proteins were identified in GRWD1 immunoprecipitations performed in low or high salt.
Curated By
- BioGRID