An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.

Watanabe S, Fujiyama H, Takafuji T, Kayama K, Matsumoto M, Nakayama KI, Yoshida K, Sugimoto N, Fujita M

Glutamate-rich WD40 repeat-containing 1 (GRWD1) is a Cdt1-binding protein that promotes mini-chromosome maintenance (MCM) loading through its histone chaperone activity. GRWD1 acts as a tumor-promoting factor by downregulating p53 (also known as TP53) via the RPL11-MDM2-p53 axis. Here, we identified GRWD1-interacting proteins using a proteomics approach and showed that GRWD1 interacts with various proteins involved in transcription, translation, DNA replication ... [more]

J. Cell. Sci. Aug. 03, 2018; 131(15); [Pubmed: 29991511]

Throughput

High Throughput

Additional Notes

The 'hit' proteins were identified in GRWD1 immunoprecipitations performed in low or high salt.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRWD1 CCT2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Raisch J (2023)	High	-	BioGRID	3569543

Curated By

BioGRID

Interaction Summary

GRWD1

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

Gene Ontology Cellular Component

CCT2

Gene Ontology Biological Process