STX4
Gene Ontology Biological Process
- blood coagulation [TAS]
- intracellular protein transport [IBA]
- long-term synaptic potentiation [IDA]
- membrane organization [TAS]
- organelle fusion [IDA]
- platelet activation [TAS]
- positive regulation of catalytic activity [IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of chemotaxis [IMP]
- positive regulation of eosinophil degranulation [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of immunoglobulin secretion [IMP]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [IDA, IMP]
- positive regulation of protein localization to cell surface [IMP]
- positive regulation of protein localization to plasma membrane [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of exocytosis [IMP]
- regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- response to hydroperoxide [IDA]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA]
- basolateral plasma membrane [IDA]
- cell surface [IDA]
- cytosol [TAS]
- dendritic spine [IDA]
- endosome [IDA]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- integral component of membrane [IBA]
- intracellular [IDA]
- lamellipodium [IDA]
- membrane [IDA]
- plasma membrane [IDA, TAS]
- somatodendritic compartment [IDA]
- specific granule [IDA]
- synapse [IDA]
- synaptic vesicle [IBA]
- vacuole [TAS]
STXBP5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation.
The Sec1p-like/Munc18 (SM) protein Munc18a binds to the neuronal t-SNARE Syntaxin1A and inhibits SNARE complex assembly. Tomosyn, a cytosolic Syntaxin1A-binding protein, is thought to regulate the interaction between Syntaxin1A and Munc18a, thus acting as a positive regulator of SNARE assembly. In the present study we have investigated the interaction between b-Tomosyn and the adipocyte SNARE complex involving Syntaxin4/SNAP23/VAMP-2 and the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX4 STXBP5 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 135 | BioGRID | 3007789 | |
| STX4 STXBP5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID