STX3
Gene Ontology Biological Process
- intracellular protein transport [IBA]
- neuron projection development [ISS]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of chemotaxis [IMP]
- positive regulation of protein localization to cell surface [IMP]
- positive regulation of protein localization to plasma membrane [IMP]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IBA, ISS]
- apical plasma membrane [IDA]
- azurophil granule [IDA]
- cell-cell junction [IDA]
- extracellular vesicular exosome [IDA]
- growth cone [ISS]
- integral component of membrane [IBA]
- lamellipodium [IDA]
- neuron projection [ISS]
- plasma membrane [IDA]
- specific granule [IDA]
- synaptic vesicle [IBA]
- vacuole [TAS]
VAMP3
Gene Ontology Biological Process
- exocytosis [TAS]
- membrane fusion [TAS]
- mucus secretion [IMP]
- negative regulation of secretion by cell [IDA]
- neurotransmitter secretion [IBA]
- positive regulation of immunoglobulin secretion [IMP]
- positive regulation of receptor recycling [ISS]
- protein complex assembly [TAS]
- regulation of histamine secretion by mast cell [IMP]
- retrograde transport, endosome to Golgi [IDA]
- substrate adhesion-dependent cell spreading [ISS]
- vesicle docking involved in exocytosis [TAS]
- vesicle fusion [IBA]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane.
Intracellular localisation of soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptors (SNAREs) is an important factor in clarifying whether SNAREs regulate exocytosis in salivary glands. We investigated intracellular localisation of syntaxins 2, 3 and 4 and SNAP-23, which are thought to be target membrane (t)-SNAREs, in rat parotid gland by Western blotting and immunocytochemistry. Syntaxins 2 and 3 were localised ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX3 VAMP3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9995 | BioGRID | 1180366 | |
| STX3 VAMP3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9978 | BioGRID | 2234762 | |
| STX3 VAMP3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9968 | BioGRID | 3047665 | |
| VAMP3 STX3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STX3 VAMP3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID