An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation.

Yue Y, Liu J, Cui X, Cao J, Luo G, Zhang Z, Cheng T, Gao M, Shu X, Ma H, Wang F, Wang X, Shen B, Wang Y, Feng X, He C, Liu J

N6-methyladenosine (m6A) is enriched in 3'untranslated region (3'UTR) and near stop codon of mature polyadenylated mRNAs in mammalian systems and has regulatory roles in eukaryotic mRNA transcriptome switch. Significantly, the mechanism for this modification preference remains unknown, however. Herein we report a characterization of the full m6A methyltransferase complex in HeLa cells identifying METTL3/METTL14/WTAP/VIRMA/HAKAI/ZC3H13 as the key components, and we ... [more]

Cell Discov Mar. 07, 2018; 4();10 [Pubmed: 29507755]

Throughput

High Throughput

Additional Notes

The RNA of the 'hit' genes was identified in an RIP-seq (RNA immunoprecipitation sequencing) experiment using the 'bait' protein, VIRMA.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KIAA1429 LRPPRC	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yue Y (2018)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

KIAA1429

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

LRPPRC

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [NAS]actin filament binding [IDA]beta-tubulin binding [IDA]microtubule binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-RNA