BAIT

DGKH

DGKeta, RP11-215B13.1
diacylglycerol kinase, eta
GO Process (4)
GO Function (1)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification and characterization of two splice variants of human diacylglycerol kinase eta.

Murakami T, Sakane F, Imai S, Houkin K, Kanoh H

Diacylglycerol kinase (DGK) participates in regulating the intracellular concentrations of two bioactive lipids, diacylglycerol and phosphatidic acid. DGK eta (eta 1, 128 kDa) is a type II isozyme containing a pleckstrin homology domain at the amino terminus. Here we identified another DGK eta isoform (eta 2, 135 kDa) that shared the same sequence with DGK eta 1 except for a ... [more]

J. Biol. Chem. Sep. 05, 2003; 278(36);34364-72 [Pubmed: 12810723]

Throughput

  • Low Throughput

Curated By

  • BioGRID