ACVR1
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- G1/S transition of mitotic cell cycle [IMP]
- activin receptor signaling pathway [IDA]
- atrial septum primum morphogenesis [IMP]
- cardiac muscle cell fate commitment [IMP]
- embryonic heart tube morphogenesis [IMP]
- endocardial cushion cell fate commitment [IMP]
- mitral valve morphogenesis [IMP]
- negative regulation of activin receptor signaling pathway [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- negative regulation of signal transduction [IMP]
- pathway-restricted SMAD protein phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of bone mineralization [IMP]
- positive regulation of determination of dorsal identity [IDA]
- positive regulation of osteoblast differentiation [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- protein phosphorylation [IDA]
- regulation of ossification [IMP]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- SMAD binding [IDA]
- activin binding [IDA]
- activin receptor activity, type I [IDA]
- peptide hormone binding [NAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- transforming growth factor beta binding [IDA]
- transmembrane receptor protein serine/threonine kinase activity [NAS]
- ATP binding [IDA]
- SMAD binding [IDA]
- activin binding [IDA]
- activin receptor activity, type I [IDA]
- peptide hormone binding [NAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- transforming growth factor beta binding [IDA]
- transmembrane receptor protein serine/threonine kinase activity [NAS]
Gene Ontology Cellular Component
SMAD1
Gene Ontology Biological Process
- BMP signaling pathway [IDA, IMP, ISS, TAS]
- SMAD protein complex assembly [IDA]
- cellular response to BMP stimulus [ISS]
- embryonic pattern specification [ISS]
- positive regulation of gene expression [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [ISS]
- primary miRNA processing [TAS]
- signal transduction [NAS]
- transforming growth factor beta receptor signaling pathway [NAS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
AMPK downregulates ALK2 via increasing the interaction between Smurf1 and Smad6, leading to inhibition of osteogenic differentiation.
Activin A receptor type I or activin receptor-like kinase 2 (ACVRI/ALK2) belongs to type I TGF-β family and plays an important role in bone development. Activating mutations of ALK2 containing the R206 to H mutation, are present in 95% in the rare autosomal genetic disease fibrodysplasia ossificans progressiva (FOP), which leads to the development of ectopic bone formation in muscle. ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using firefly luciferase complementation assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ACVR1 SMAD1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ACVR1 SMAD1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID