BAIT

YDCY

ECK1440, JW1441, b1446
DUF2526 family protein
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/MG1655)
PREY

FIXX

ECK0045, JW0043, yaaT, b0044
putative 4Fe-4S ferredoxin-type protein
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/MG1655)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The binary protein-protein interaction landscape of Escherichia coli.

Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Haeuser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P

Efforts to map the Escherichia coli interactome have identified several hundred macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not been surveyed on a large scale. Here we performed yeast two-hybrid screens of 3,305 baits against 3,606 preys (∼70% of the E. coli proteome) in duplicate to generate a map of 2,234 interactions, which approximately doubles the number of ... [more]

Nat. Biotechnol. Mar. 01, 2014; 32(3);285-90 [Pubmed: 24561554]

Throughput

  • High Throughput

Curated By

  • BioGRID