BAIT

PTPN11

2700084A17Rik, AW536184, PTP1D, PTP2C, SAP-2, SH-PTP2, SH-PTP3, SHP-2, Shp2, Syp
protein tyrosine phosphatase, non-receptor type 11
GO Process (41)
GO Function (12)
GO Component (8)

Gene Ontology Biological Process

Mus musculus
PREY

IL6ST

5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
interleukin 6 signal transducer
GO Process (20)
GO Function (12)
GO Component (12)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association.

Stofega MR, Wang H, Ullrich A, Carter-Su C

SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domain-containing protein-tyrosine phosphatase SHP-2 in response to insulin. Here we examine whether SIRPalpha and SHP-2 are signaling molecules for the receptors for growth hormone (GH), leukemia inhibitory factor (LIF), or interferon-gamma (IFNgamma), cytokine receptor superfamily members that bind to and ... [more]

J. Biol. Chem. Mar. 20, 1998; 273(12);7112-7 [Pubmed: 9507023]

Throughput

  • Low Throughput

Curated By

  • BioGRID