An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

E3 ubiquitin ligase HECW2 targets PCNA and lamin B1.

Krishnamoorthy V, Khanna R, Parnaik VK

Lamins constitute the major architectural proteins of the nuclear lamina that help in maintaining nuclear organization. Mutations in lamins are associated with diverse degenerative diseases, collectively termed laminopathies. HECW2, a HECT-type E3 ubiquitin ligase, is transcriptionally upregulated in HeLa cells expressing Emery-Dreifuss muscular dystrophy-causing-lamin A mutants. However, the role of HECW2 upregulation in mediating downstream effects in lamin mutant-expressing cells ... [more]

Biochim Biophys Acta Mol Cell Res Dec. 01, 2017; 1865(8);1088-1104 [Pubmed: 29753763]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HECW2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IBA]

Gene Ontology Cellular Component

LMNA