BAIT

APC

BTPS2, DP2, DP2.5, DP3, GS, PPP1R46
adenomatous polyposis coli
GO Process (21)
GO Function (8)
GO Component (12)
Homo sapiens
PREY

ASAP2

AMAP2, CENTB3, DDEF2, PAG3, PAP, Pap-alpha, SHAG1
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of a link between the SAMP repeats of adenomatous polyposis coli tumor suppressor and the Src homology 3 domain of DDEF.

Matsui C, Kaieda S, Ikegami T, Mimori-Kiyosue Y

The adenomatous polyposis coli (APC) tumor suppressor protein is a multifunctional protein with a well characterized role in the Wnt signal transduction pathway and in cytoskeletal regulation. The SAMP repeats region of APC, an Axin-binding site, is known to be important for tumor suppression and for the developmental function of APC. We performed a yeast two-hybrid screening using the first ... [more]

J. Biol. Chem. Nov. 21, 2008; 283(47);33006-20 [Pubmed: 18786926]

Throughput

  • Low Throughput

Curated By

  • BioGRID