BAIT

ERLIN2

C8orf2, Erlin-2, NET32, SPFH2, SPG18, UNQ2441
ER lipid raft associated 2
Alzheimer's Disease Project
UPS Project
GO Process (1)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

RNF170

6720407G21Rik, AI481227
ring finger protein 170
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The erlin2 T65I mutation inhibits erlin1/2 complex-mediated inositol 1,4,5-trisphosphate receptor ubiquitination and phosphatidylinositol 3-phosphate binding.

Wright FA, Bonzerato CG, Sliter DA, Wojcikiewicz RJH

The erlin1/2 complex is a ∼2-MDa endoplasmic reticulum membrane-located ensemble of the ∼40-kDa type II membrane proteins erlin1 and erlin2. The best defined function of this complex is to mediate the ubiquitination of activated inositol 1,4,5-trisphosphate receptors (IP3Rs) and their subsequent degradation. However, it remains unclear how mutations of the erlin1/2 complex affect its cellular function and cause cellular dysfunction ... [more]

J. Biol. Chem. Dec. 05, 2017; 293(40);15706-15714 [Pubmed: 30135210]

Throughput

  • Low Throughput

Curated By

  • BioGRID