Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DEPDC1B coordinates de-adhesion events and cell-cycle progression at mitosis.

Marchesi S, Montani F, Deflorian G, D'Antuono R, Cuomo A, Bologna S, Mazzoccoli C, Bonaldi T, Di Fiore PP, Nicassio F

Cells entering mitosis become rounded, lose attachment to the substrate, and increase their cortical rigidity. Pivotal to these events is the dismantling of focal adhesions (FAs). How mitotic reshaping is linked to commitment to divide is unclear. Here, we show that DEPDC1B, a protein that accumulates in G2, coordinates de-adhesion events and cell-cycle progression at mitosis. DEPDC1B functions as an ... [more]

Dev. Cell Nov. 24, 2014; 31(4);420-33 [Pubmed: 25458010]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PTPRF

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein tyrosine phosphatase activity [IDA, IMP]transmembrane receptor protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

EEF1G