An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer.

Bhuripanyo K, Wang Y, Liu X, Zhou L, Liu R, Duong D, Zhao B, Bi Y, Zhou H, Chen G, Seyfried NT, Chazin WJ, Kiyokawa H, Yin J

E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins and regulate diverse cellular processes. To profile their ubiquitination targets in the cell, we used phage display to engineer E2-E4B and E2-CHIP pairs that were free of cross-reactivity with the native ... [more]

Sci Adv Dec. 01, 2017; 4(1);e1701393 [Pubmed: 29326975]

Throughput

Low Throughput

Additional Notes

in vitro ubiquitination with UBE1 as E1, UbcH5b as E2, E4B as E3 and the indicated hit protein(s) as substrate

Curated By

BioGRID

Interaction Summary

UBE4B

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [ISS]

Gene Ontology Cellular Component

PGAM5