UBE4B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PGAM5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer.
E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins and regulate diverse cellular processes. To profile their ubiquitination targets in the cell, we used phage display to engineer E2-E4B and E2-CHIP pairs that were free of cross-reactivity with the native ... [more]
Throughput
- Low Throughput
Additional Notes
- in vitro ubiquitination with UBE1 as E1, UbcH5b as E2, E4B as E3 and the indicated hit protein(s) as substrate
Curated By
- BioGRID