PRDX1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UCHL1
Gene Ontology Biological Process
- adult walking behavior [ISO]
- axon target recognition [ISO]
- axon transport of mitochondrion [ISO]
- axonogenesis [ISO]
- cell proliferation [ISO]
- eating behavior [ISO]
- muscle fiber development [ISO]
- negative regulation of MAP kinase activity [ISO]
- neuromuscular process [ISO]
- protein deubiquitination [ISO]
- response to ischemia [ISO]
- sensory perception of pain [IDA]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Structural and biochemical analyses reveal ubiquitin C-terminal hydrolase-L1 as a specific client of the peroxiredoxin II chaperone.
Peroxiredoxins (Prxs) play dual roles as both thiol-peroxidases and molecular chaperones. Peroxidase activity enables various intracellular functions, however, the physiological roles of Prxs as chaperones are not well established. To study the chaperoning function of Prx, we previously sought to identify heat-induced Prx-binding proteins as the clients of a Prx chaperone. By using His-tagged Prx I as a bait, we ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using Ni-NTA pull-down of His-tagged proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRDX1 UCHL1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UCHL1 PRDX1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UCHL1 PRDX1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2574772 |
Curated By
- BioGRID