Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.

Viita T, Kyheroeinen S, Prajapati B, Virtanen J, Frilander MJ, Varjosalo M, Vartiainen MK

In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanisms by which actin operates in the nucleus remain poorly understood. Here, we have used two complementary mass spectrometry (MS) techniques, AP-MS and BioID, ... [more]

J. Cell. Sci. Apr. 17, 2019; 132(8); [Pubmed: 30890647]

Throughput

  • High Throughput

Curated By

  • BioGRID