BAIT
RPN-11
CELE_K07D4.3, K07D4.3
rpn-11 encodes a predicted non-ATPase subunit of the 19S regulatory complex of the proteasome that affects adult viability, osmoregulation, embryonic viability, and movement; interacts with itself, RPN-9, and F55A11.3, based on yeast two-hybrid assays.
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
DAF-21
CELE_C47E8.5, tax-3, C47E8.5
daf-21 encodes a member of the Hsp90 family of molecular chaperones; DAF-21 activity is required for larval development, negative regulation of dauer larva formation, and a number of specific chemosensory behaviors, such as the response to chemicals and odorants detected by the ASE and AWC sensory neurons; a daf-21 sensory defect can be rescued by a cyclic GMP analogue, suggesting that, in regulating chemosensory behaviors, DAF-21 acts at a similar point as the DAF-11 guanylyl cyclase; down-regulation of daf-21 via RNAi results in a small but reproducible reduction in age-1 life span, suggesting that daf-21 is part of a chaperone network required for the extended life span seen in age-1 mutant animals.
GO Process (12)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- apoptotic process [IMP]
- dauer larval development [IGI, IMP]
- defense response [IMP]
- determination of adult lifespan [IGI, IMP]
- embryo development ending in birth or egg hatching [IMP]
- gonad development [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- nematode larval development [IMP]
- protein phosphorylation [IMP]
- reproduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
EPIC: software toolkit for elution profile-based inference of protein complexes.
Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectrometry analysis (chromatographic fractionation-mass spectrometry, CF-MS), but the resulting data are challenging to process and interpret. Here, we describe EPIC ... [more]
Nat. Methods Jul. 15, 2019; (); [Pubmed: 31308550]
Quantitative Score
- 0.534 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- High confidence protein interactions have an EPIC PPI score >= 0.5
Curated By
- BioGRID