PKD2L1
Gene Ontology Biological Process
- cation transport [ISS]
- cellular response to acidic pH [ISS]
- detection of chemical stimulus involved in sensory perception of sour taste [ISS]
- detection of mechanical stimulus [IBA]
- potassium ion transmembrane transport [IDA]
- protein homotrimerization [IDA]
- sensory perception of sour taste [IMP]
- smoothened signaling pathway [ISS]
- sodium ion transmembrane transport [IDA]
Gene Ontology Molecular Function- alpha-actinin binding [IPI]
- calcium activated cation channel activity [IDA]
- calcium channel activity [IBA, IDA]
- calcium ion binding [IDA, ISS]
- calcium-activated potassium channel activity [IDA]
- cation channel activity [IDA]
- cation transmembrane transporter activity [ISS]
- cytoskeletal protein binding [IPI]
- identical protein binding [ISS]
- muscle alpha-actinin binding [IPI]
- protein binding [IPI]
- sodium channel activity [IDA]
- sour taste receptor activity [ISS]
- alpha-actinin binding [IPI]
- calcium activated cation channel activity [IDA]
- calcium channel activity [IBA, IDA]
- calcium ion binding [IDA, ISS]
- calcium-activated potassium channel activity [IDA]
- cation channel activity [IDA]
- cation transmembrane transporter activity [ISS]
- cytoskeletal protein binding [IPI]
- identical protein binding [ISS]
- muscle alpha-actinin binding [IPI]
- protein binding [IPI]
- sodium channel activity [IDA]
- sour taste receptor activity [ISS]
Gene Ontology Cellular Component
TNNI3
Gene Ontology Biological Process
- cardiac muscle contraction [IMP]
- cellular calcium ion homeostasis [ISS]
- heart contraction [IMP]
- heart development [ISS]
- muscle filament sliding [TAS]
- negative regulation of ATPase activity [IDA, IMP]
- regulation of systemic arterial blood pressure by ischemic conditions [ISS]
- vasculogenesis [ISS]
- ventricular cardiac muscle tissue morphogenesis [IMP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Troponin I binds polycystin-L and inhibits its calcium-induced channel activation.
Polycystin-L (PCL) is an isoform of polycystin-2, the product of the second gene associated with autosomal dominant polycystic kidney disease, and functions as a Ca(2+)-regulated nonselective cation channel. We recently demonstrated that polycystin-2 interacts with troponin I, an important regulatory component of the actin microfilament complex in striated muscle cells and an angiogenesis inhibitor. In this study, using the two-microelectrode ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PKD2L1 TNNI3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PKD2L1 TNNI3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID