BAIT

DAB2

DOC-2, DOC2
Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
GO Process (23)
GO Function (5)
GO Component (9)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of the Wnt signaling pathway by disabled-2 (Dab2).

Hocevar BA, Mou F, Rennolds JL, Morris SM, Cooper JA, Howe PH

The adaptor molecule Disabled-2 (Dab2) has been shown to link cell surface receptors to downstream signaling pathways. Using a small-pool cDNA screening strategy, we identify that the N-terminal domain of Dab2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of Dab2 in NIH-3T3 mouse fibroblasts attenuates canonical Wnt/beta-catenin-mediated signaling, including accumulation of beta-catenin, activation of ... [more]

EMBO J. Jun. 16, 2003; 22(12);3084-94 [Pubmed: 12805222]

Throughput

  • Low Throughput

Curated By

  • BioGRID