An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

GAP43 stimulates inositol trisphosphate-mediated calcium release in response to hypotonicity.

Caprini M, Gomis A, Cabedo H, Planells-Cases R, Belmonte C, Viana F, Ferrer-Montiel A

The identification of osmo/mechanosensory proteins in mammalian sensory neurons is still elusive. We have used an expression cloning approach to screen a human dorsal root ganglion cDNA library to look for proteins that respond to hypotonicity by raising the intracellular Ca(2+) concentration ([Ca(2+)](i)). We report the unexpected identification of GAP43 (also known as neuromodulin or B50), a membrane-anchored neuronal protein ... [more]

EMBO J. Jun. 16, 2003; 22(12);3004-14 [Pubmed: 12805215]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GAP43

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

PLCD1