BAIT
SEC14
PIT1, phosphatidylinositol/phosphatidylcholine transfer protein SEC14, L000001839, YMR079W
Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
GO Process (8)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [IMP]
- Golgi to vacuole transport [IGI, IMP]
- Golgi vesicle budding [IDA]
- ascospore formation [IMP]
- negative regulation of phosphatidylcholine biosynthetic process [IDA, IMP]
- negative regulation of phosphatidylglycerol biosynthetic process [IMP]
- phosphatidylinositol metabolic process [IGI, IMP]
- phospholipid transport [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RPL9A
ribosomal 60S subunit protein L9A, L6, rp24, YL11, L9A, L8A, L000001708, YGL147C
Ribosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The oxysterol binding protein Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function.
The Saccharomyces cerevisiae phosphatidylcholine/phosphatidylinositol transfer protein Sec14p is required for Golgi apparatus-derived vesicular transport through coordinate regulation of phospholipid metabolism. Sec14p is normally essential. The essential requirement for SEC14 can be bypassed by inactivation of (i) the CDP-choline pathway for phosphatidylcholine synthesis or (ii) KES1, which encodes an oxysterol binding protein. A unique screen was used to determine genome-wide genetic ... [more]
Proc. Natl. Acad. Sci. U.S.A. Sep. 25, 2007; 104(39);15352-7 [Pubmed: 17881569]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- An SGA screen was used to identify genes specifically required for viability of the query strain which was a sec14 cki1 double mutant.
Curated By
- BioGRID