BAIT

SEC14

PIT1, phosphatidylinositol/phosphatidylcholine transfer protein SEC14, L000001839, YMR079W
Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

KEX2

QDS1, SRB1, VMA45, kexin KEX2, yscF, L000000896, YNL238W
Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The oxysterol binding protein Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function.

Fairn GD, Curwin AJ, Stefan CJ, McMaster CR

The Saccharomyces cerevisiae phosphatidylcholine/phosphatidylinositol transfer protein Sec14p is required for Golgi apparatus-derived vesicular transport through coordinate regulation of phospholipid metabolism. Sec14p is normally essential. The essential requirement for SEC14 can be bypassed by inactivation of (i) the CDP-choline pathway for phosphatidylcholine synthesis or (ii) KES1, which encodes an oxysterol binding protein. A unique screen was used to determine genome-wide genetic ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 25, 2007; 104(39);15352-7 [Pubmed: 17881569]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • An SGA screen was used to identify genes specifically required for viability of the query strain which was a sec14 cki1 double mutant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SEC14 KEX2
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
343859

Curated By

  • BioGRID