BAIT
CLEC-63
CELE_F35C5.6, F35C5.6
C-type LECtin
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans
PREY
ACO-1
CELE_ZK455.1, gei-22, ZK455.1
aco-1 encodes an aconitase that is homologous to mammalian iron regulatory protein-1 (IRP1); aco-1 activity is required for normal brood sizes and, under iron stress conditions, for normal lifespan and L4-to-adult growth rates; ACO-1 physically interacts with GEX-3; like IRP1, ACO-1 has aconitase activity and is post-translationally regulated by iron, but, unlike IRP1, it lacks RNA-binding activity; aco-1 mRNA levels appear to decrease in response to iron treatment; an ACO-1::GFP fusion protein exhibits cytosolic expression in the hypodermal seam cells and the intestine.
GO Process (2)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Caenorhabditis elegans
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
EPIC: software toolkit for elution profile-based inference of protein complexes.
Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectrometry analysis (chromatographic fractionation-mass spectrometry, CF-MS), but the resulting data are challenging to process and interpret. Here, we describe EPIC ... [more]
Nat. Methods Jul. 15, 2019; (); [Pubmed: 31308550]
Quantitative Score
- 0.559 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- High confidence protein interactions have an EPIC PPI score >= 0.5
Curated By
- BioGRID