PREY

RABIF

MSS4, RASGFR3, RASGRF3
RAB interacting factor
GO Process (2)
GO Function (3)
GO Component (0)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

MICAL-1 isoforms, novel rab1 interacting proteins.

Weide T, Teuber J, Bayer M, Barnekow A

Rab1 GTPases participate in regulating the vesicular transport of ER-Golgi compartments. Recently, GM130, p115, and Golgin-84 were identified as effectors of the active conformation of rab1. Here, we describe a novel protein, MICAL-1b, a splice variant of the MICAL-1a protein. Using the yeast two-hybrid system, we showed that it specifically interacts with rab1 in a nucleotide-dependent manner. The interaction was ... [more]

Biochem. Biophys. Res. Commun. Jun. 20, 2003; 306(1);79-86 [Pubmed: 12788069]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RABIF RAB1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9959BioGRID
3191842
RAB1A RABIF
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9852BioGRID
3098407
RAB1A RABIF
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2726481

Curated By

  • BioGRID