An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis.

Rosse C, L'Hoste S, Offner N, Picard A, Camonis J

The Ral signaling pathway is critically involved in Ras-dependent oncogenesis. One of its key actors, RLIP/RalBP1, which participates in receptor endocytosis during interphase, is also involved in mitotic processes when endocytosis is switched off. During mitosis, RLIP76 is located on the duplicated centrosomes and is required for their proper separation and movement to the poles. We have looked for actors ... [more]

J. Biol. Chem. Aug. 15, 2003; 278(33);30597-604 [Pubmed: 12775724]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RALBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]ATPase activity, coupled to movement of substances [IDA]GTPase activator activity [IDA]Rac GTPase activator activity [IDA]Rac GTPase binding [IPI]Ral GTPase binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

NUMB