BAIT

ERAS

HRAS2, HRASP
ES cell expressed Ras
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

PIK3CG

PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.

Takahashi K, Mitsui K, Yamanaka S

Embryonic stem (ES) cells are pluripotent cells derived from early mammalian embryos. Their immortality and rapid growth make them attractive sources for stem cell therapies; however, they produce tumours (teratomas) when transplanted, which could preclude their therapeutic usage. Why ES cells, which lack chromosomal abnormalities, possess tumour-like properties is largely unknown. Here we show that mouse ES cells specifically express ... [more]

Nature May. 29, 2003; 423(6939);541-5 [Pubmed: 12774123]

Throughput

  • Low Throughput

Curated By

  • BioGRID