RHOA
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- Rho protein signal transduction [TAS]
- actin cytoskeleton organization [TAS]
- apical junction assembly [IMP]
- apolipoprotein A-I-mediated signaling pathway [IMP]
- axon guidance [TAS]
- blood coagulation [TAS]
- cleavage furrow formation [IDA]
- negative chemotaxis [IMP]
- negative regulation of axonogenesis [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- ossification involved in bone maturation [ISS]
- phosphatidylinositol-mediated signaling [TAS]
- platelet activation [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IEP]
- positive regulation of NF-kappaB import into nucleus [NAS]
- positive regulation of axonogenesis [TAS]
- positive regulation of cytokinesis [IMP]
- positive regulation of neuron differentiation [IMP]
- positive regulation of stress fiber assembly [IDA]
- regulation of axonogenesis [TAS]
- regulation of cell migration [IMP]
- regulation of osteoblast proliferation [ISS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- spindle assembly involved in mitosis [IMP]
- substantia nigra development [IEP]
- trabecula morphogenesis [ISS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PKN1
Gene Ontology Biological Process
Gene Ontology Molecular Function- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
RhoE binds to ROCK I and inhibits downstream signaling.
RhoE belongs to the Rho GTPase family, the members of which control actin cytoskeletal dynamics. RhoE induces stress fiber disassembly in a variety of cell types, whereas RhoA stimulates stress fiber assembly. The similarity of RhoE and RhoA sequences suggested that RhoE might compete with RhoA for interaction with its targets. Here, we show that RhoE binds ROCK I but ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RHOA PKN1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low/High | - | BioGRID | 3328664 | |
| PKN1 RHOA | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | BioGRID | - | |
| PKN1 RHOA | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| PKN1 RHOA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| PKN1 RHOA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RHOA PKN1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RHOA PKN1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID