Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors.

Chuang TH, Ulevitch RJ

Activation of Toll-like receptors (TLRs) results in a proinflammatory response needed to combat infection. Thus, limiting TLR signaling is essential for preventing a protective response from causing injury to the host. Here we describe how a RING finger protein, Triad3A, acts as an E3 ubiquitin-protein ligase and enhances ubiquitination and proteolytic degradation of some TLRs. Triad3A overexpression promoted substantial degradation ... [more]

Nat. Immunol. May. 01, 2004; 5(5);495-502 [Pubmed: 15107846]


  • Low Throughput

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  • BioGRID