Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of E-box factor TFE3 as a functional partner for the E2F3 transcription factor.

Giangrande PH, Hallstrom TC, Tunyaplin C, Calame K, Nevins JR

Various studies have demonstrated a role for E2F proteins in the control of transcription of genes involved in DNA replication, cell cycle progression, and cell fate determination. Although it is clear that the functions of the E2F proteins overlap, there is also evidence for specific roles for individual E2F proteins in the control of apoptosis and cell proliferation. Investigating protein ... [more]

Mol. Cell. Biol. Jun. 01, 2003; 23(11);3707-20 [Pubmed: 12748276]

Throughput

  • Low Throughput

Curated By

  • BioGRID