CD44
Gene Ontology Biological Process
- blood coagulation [TAS]
- carbohydrate metabolic process [TAS]
- cartilage development [IEP]
- cell-matrix adhesion [NAS]
- cellular response to fibroblast growth factor stimulus [IDA]
- cytokine-mediated signaling pathway [TAS]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- glycosaminoglycan metabolic process [TAS]
- hyaluronan catabolic process [IDA, TAS]
- hyaluronan metabolic process [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- leukocyte migration [TAS]
- monocyte aggregation [IMP]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of heterotypic cell-cell adhesion [IMP]
- positive regulation of monocyte aggregation [IMP]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- single organismal cell-cell adhesion [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARHGEF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression.
In this study we have examined CD44 (a hyaluronan (HA) receptor) interaction with a RhoA-specific guanine nucleotide exchange factor (p115RhoGEF) in human metastatic breast tumor cells (MDA-MB-231 cell line). Immunoprecipitation and immunoblot analyses indicate that both CD44 and p115RhoGEF are expressed in MDA-MB-231 cells and that these two proteins are physically associated as a complex in vivo. The binding of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARHGEF1 CD44 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID