An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphacan short isoform, a novel non-proteoglycan variant of phosphacan/receptor protein tyrosine phosphatase-beta, interacts with neuronal receptors and promotes neurite outgrowth.

Garwood J, Heck N, Reichardt F, Faissner A

Phosphacan, one of the principal proteoglycans in the extracellular matrix of the central nervous system, is implicated in neuron-glia interactions associated with neuronal differentiation and myelination. We report here the identification of a novel truncated form of phosphacan, phosphacan short isoform (PSI), that corresponds to the N-terminal carbonic anhydrase- and fibronectin type III-like domains and half of the spacer region. ... [more]

J. Biol. Chem. Jun. 27, 2003; 278(26);24164-73 [Pubmed: 12700241]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PTPRZ1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine phosphatase activity [ISS]transmembrane receptor protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

CNTN1